Membrane:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Labeling:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Recombinant:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Plasmid Preparation:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Software:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Microscopy:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
Flow Cytometry:Article Title: Valrubicin-loaded immunoliposomes targeting antigens on immunosuppressive cells to circumvent resistance to cancer immunotherapy.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Vector NovaRED substrate kit Vector Laboratories Cat# SK-4800 PKH67 Green Fluorescent Cell Linker Mini Kit for General Cell Membrane Labeling Merck Cat# MINI67 MycoAlert Detection Kit Lonza-Bioscience Cat# LT07 10% buffered formalin (CellStor Pot) Cellpath Cat# BAF-6000-08A Experimental models: Cell lines Murine T lymphoma EL4 cells ATCC Cat# TIB-39 Murine B lymphoma A20 cells ATCC Cat# TIB-208 Murine breast cancer 4T1 cells ATCC Cat# CRL-2539 Murine Lewis lung carcinoma LLC1 (LL/2) cells ATCC Cat# CRL-1642 Human embryonic kidney (HEK) 293T cells ATCC Cat# CRL-3216 Experimental models: Organisms/strains Mouse: C57BL/6 Envigo N/A Mouse: BALB/c Janvier N/A Recombinant DNA pCCLc-MNDU3-Luciferase-PGK-EGFPWPRE plasmid vector Addgene Cat# 89608; RRID: Addgene_89608 PAX2 plasmid vector Addgene Cat# 12260; RRID: Addgene_12260 pCMV-VSV-G plasmid vector Addgene Cat# 8454; RRID: Addgene_8454 Software and algorithms FlowJo (v. 10.8.1) Tree Star https://www.flowjo.com UMAP plugin Tree Star https://www.flowjo.com FlowSOM plugin Tree Star https://www.flowjo.com GraphPad Prism (v. 10.2.3) GraphPad https://www.graphpad.com Living Image software (v. 4.0) PerkinElmer https://www.perkinelmer.com Zen software (v. 3.9) Zeiss https://www.zeiss.com/microscopy BioRender BioRender https://www.biorender.com GIMP (v. 3.0.4) GIMP https://www.gimp.org Other Val-ILs nanoparticles This paper N/A 300 kDa dialysis membranes Thermo Fisher Scientific Cat# 11550970 Amicon Centrifugal Filter Unit 100 kDa cutoff Millipore Cat# UFC910096 AxioScope microscope Zeiss https://www.zeiss.com/microscopy Aurora flow cytometer Cytek https://cytekbio.com/pages/aurora Videodrop Myriade Lab https://www.myriadelab.com Hemocytometer (Vet ABC+). .. SCIL https://www.scilvet.fr/produits/diagnostic-de- laboratoire/hematologie/scil-vet-abc-plus NanoSight NS300 Malvern Panalytical https://www.malvernpanalytical.com e4 Cell Reports Medicine 7, 102632, March 17, 2026 OPEN ACCESS into the flanks of C57BL/6 mice (Envigo) or BALB/c mice (Janvier), respectively.
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